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. 2004 Jun;164(6):2003–2012. doi: 10.1016/s0002-9440(10)63760-1

Figure 1.

Figure 1

ER-β 5′-flanking region (AF191544) structure and primer design for bisulfite sequencing. A: Two CGIs (island 1, −615 to −455; island 2, −362 to −108) were identified. Individual CpG sites are indicated as vertical lines and numbered from 1 to 41. Island 1 contains CpG sites 1 to 15 located in the promoter and island 2 contains CpG sites 16 to 41 located in exon 0N. A 10,958-bp intron region between exon 0N and exon 1 (AL161756) is indicated by a black arrowhead. The 500-bp nested PCR-amplified region is indicated by a line. B: Sequence of ER-β promoter, exon 0N, and exon 1 region. The 5′ transcription start site is designated as + 1. The start site of exon 0N is indicated by a bent arrow. Potential transcription factor binding sites are indicated. The two round primers for PCR are dark-blocked.