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. 2004 Jun;164(6):2039–2054. doi: 10.1016/S0002-9440(10)63763-7

Figure 6.

Figure 6

Effect of PRL-3 expression on B16 melanoma cell adhesion to various matrix proteins. Mock vector-transfected and vector/PRL-3-transfected cells were added to the wells of 96-well plates coated with fibronectin (10 μg/ml) (A), laminin (10 μg/ml) (B), or collagen I (50 μg/ml) (C). After incubation at 37°C for 30 minutes, nonadherent cells were gently washed away, and the amount of the cell adhesion was determined using an enzyme-linked immunosorbent assay reader after addition of MTT as indicated in Materials and Methods. For anti-sense ODN treatment, the cells were transfected with 500 nmol/L of ODNs before adhesion assay. Normal, untreated cells; reagent, treated with oligofectamine only; AS, PRL-3 anti-sense ODN; S, scrambled ODN; control, randomized ODN. Data are mean ± SEM of three independent experiments and each experiment includes triplicate sets. **, P < 0.01, versus mock vector; #, P < 0.05; ##, P < 0.01, versus reagent.