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. 2004 Jun;164(6):2241–2249. doi: 10.1016/S0002-9440(10)63780-7

Figure 3.

Figure 3

A: Northern blot analysis of GAPDH and eNOS mRNA in sham-operated control lung tissue (sham) and in lung tissue exposed to 1 hour of ischemia followed by 24 hours of reperfusion (group C). Experiments were performed in WT animals. B: Detection of GAPDH (357 bp) and eNOS (292 bp) transcripts by RT-PCR in RNA extracted from sham-operated control lungs (sham) and left lungs exposed to 1 hour of ischemia, followed by either 0.5 hours of reperfusion (group A), 5 hours of reperfusion (group B), or 24 hours of reperfusion (group C). Experiments were performed in WT animals. C: Densitometric assessment of pulmonary eNOS to GAPDH transcript ratio in sham-operated control lungs (sham) and left lungs exposed to 1 hour of ischemia, followed by either 0.5 hours of reperfusion (group A), 5 hours of reperfusion (group B), or 24 hours of reperfusion (group C). Experiments were performed in WT animals. Mean ± SD, * P < 0.05 versus sham. D: Western blot analysis of eNOS protein in sham-operated control lung tissue (sham) and in lung tissue exposed to 1 hour of ischemia followed by 24 hours of reperfusion (group C). Experiments were performed in WT animals.