Immunohistochemical staining of CD18-positive leukocytes in pulmonary tissue from sham-operated lungs (sham, A) and left lungs exposed to 1 hour of ischemia, followed by either 0.5 hours of reperfusion (group A, B), 5 hours of reperfusion (group B, C), or 24 hours of reperfusion (group C, D). Magnification, ×200.