Figure 7.

A: Detection of GAPDH (357 bp), ICAM-1 (437 bp) and VCAM (404 bp) transcripts by RT-PCR in RNA extracted from sham-operated lungs (sham) and left lungs exposed to 1 hour of ischemia, followed by either 0.5 hours of reperfusion (group A), 5 hours of reperfusion (group B), or 24 hours of reperfusion (group C). Samples with H₂O instead of DNA template were run to exclude DNA contamination. Experiments were performed in WT animals. B: Detection of GAPDH (357 bp), ICAM-1 (437 bp), and VCAM (404 bp) transcripts by RT-PCR in RNA extracted from sham-operated lungs (sham) and left lungs exposed to 1 hour of ischemia, followed by either 0.5 hours of reperfusion (group A), 5 hours of reperfusion (group B), or 24 hours of reperfusion (group C). Samples with H₂O instead of DNA template were run to exclude DNA contamination. Experiments were performed in eNOS-KO animals.