Figure 7.

Cleavage of the CFP-LEVD-YFP FRET probe and its relationship to annexin V binding in Jurkat cells, including wild-type (A), caspase 8-deficient (B) or those stably transfected with bcl-x (C). Cells were transfected with the CFP-LEVD-YFP probe and then stimulated with anti-Fas antibody or staurosporine (STS). Distinct regions R2 (in red) and R3 (in green) were gated for the FRET-positive population and a population with diminished FRET, respectively. The gated annexin V-APC histogram of each population (green, R3; red, R2) is shown in each panel.