Alignment of deduced amino acid sequences of the ORF3 translational product of ISPpa5, ISRm15 of S. meliloti, ISPre3 of P. resinovorans, ISEco8 of E. coli, and two unnamed IS66-like elements of Ruegeria sp. (Rueg.) and M. loti. Dots indicate gaps introduced to maximize the alignment. Amino acids identical to the protein of ISPpa5 are boldface and shaded. The four conserved DDEE residues (encompassing the putative DDE motif), distinguished on the basis of comparative studies of 42 related sequences, are shown on a black background. Amino acid numbering is shown to the right of the sequence lines.