TABLE 2.
Lac+ mutation rate determinations from controlled-time colony counts
| dinB allelea and expt | Mutation rate (no. of mutations/ cell/generation) | Mean mutation rate (±SEM) (no. of mutations/cell/ generation) | P value for difference from dinB+b |
|---|---|---|---|
| dinB+ | |||
| 1 | 11 × 10−10 | 9.4 (±1) × 10−10 | |
| 2 | 8.2 × 10−10 | ||
| 3 | 12 × 10−10 | ||
| 4 | 8.9 × 10−10 | ||
| 5 | 16 × 10−10 | ||
| 6 | 11 × 10−10 | ||
| 7 | 12 × 10−10 | ||
| 8 | 5.8 × 10−10 | ||
| 9 | 5.7 × 10−10 | ||
| 10 | 5.2 × 10−10 | ||
| 11 | 7.6 × 10−10 | ||
| dinB10 | |||
| 1 | 6.6 × 10−10 | 8.2 (±1) × 10−10 | 0.97 |
| 2 | 7.9 × 10−10 | ||
| 3 | 10 × 10−10 | ||
| Δ(dinB-yafN)::kan | |||
| 1 | 3.5 × 10−10 | 5.6 (±0.3) × 10−10 | 0.025 |
| 2 | 5.8 × 10−10 | ||
| 3 | 5.8 × 10−10 | ||
| 4 | 6.8 × 10−10 | ||
| 5 | 6.6 × 10−10 | ||
| 6 | 6.0 × 10−10 | ||
| 7 | 5.5 × 10−10 | ||
| 8 | 4.3 × 10−10 | ||
| 9 | 4.6 × 10−10 | ||
| 10 | 5.4 × 10−10 | ||
| 11 | 7.2 × 10−10 | ||
| ΔdinB51 | |||
| 4 | 9.0 × 10−10 | 10 (±1) × 10−10 | 0.98 |
| 5 | 8.4 × 10−10 | ||
| 6 | 12 × 10−10 | ||
| 7 | 12 × 10−10 | ||
| Δ(yafN-yafP)602 | |||
| 4 | 6.2 × 10−10 | 8.7 (±1) × 10−10 | 0.96 |
| 5 | 6.8 × 10−10 | ||
| 6 | 3.4 × 10−10 | ||
| 7 | 17 × 10−10 | ||
| 8 | 11 × 10−10 | ||
| 9 | 5.6 × 10−10 | ||
| 10 | 9.3 × 10−10 | ||
| 11 | 10 × 10−10 |
a
The strains assayed were SMR4562, SMR5830, SMR6111, SMR5889, and SMR7491, and the Lac+ timing control strains for them, plated in parallel, were SMR3804, SMR3805, SMR3807, SMR3855 to SMR3859, SMR3861, SMR3863, SMR5865 to SMR5874, SMR6356 to SMR6365, SMR7481 to SMR7490, and SMR7492 to SMR7501 (Table 1).
b
P values were calculated by using single-factor ANOVA and the Tukey test (31) with SigmaStat 2.03 for Windows by SPSS, Inc., with the mutation rates at three significant figures.