Figure 1.
Effect of Tranilast on proliferation of HaCaT cells. HaCaT cells (1 × 104 per well) in 96-well plates cultured overnight were treated with growth media containing Tranilast (0, 30, 100, 300, or 1000 μmol l−1 (μM)). Cell number was determined by MTT assay. Tranilast is seen to inhibit the proliferation of HaCaT cells in a dose-dependent manner. Cytotoxicity manifested as LDH level was also determined under the effects of Tranilast. Each value represents the mean±s.d. (n=6). ***P<0.001 as compared to DMSO control in each group.