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. 2005 Nov 14;147(1):117–124. doi: 10.1038/sj.bjp.0706460

Figure 3.

Figure 3

Mutational analysis of the p21 promoter. Mutants #1–#6 were generated from different site-directed mutagenesis of the p21p93-S sequence. HaCaT cells were transfected with these mutants and then treated with TGF-β1 or Tranilast. The responses to TGF-β1 and Tranilast were almost identical. The sequences between −74 and −83 bp, a previously defined TGF-β-response element (TβRE), were essential to both TGF-β1- and Tranilast-induced activation. Each value represents the mean±s.d. (n=6). *P<0.05; **P<0.01 as compared to DMSO control in each group.