TABLE 3.

Influence of various mutations on efficiency of sporulation

Strain	Genotype	Sporulation (CFU/ml)a		% Sporu- lation
		Viable cells	Heat- resistant cells
MB24	Wild type	5.9 × 108	3.5 × 108	59.0
JOB44	spoIIIJ::km	1.8 × 108	1.0 × 102	<0.001
JOB20	spoIIIJ::sp	3.0 × 108	1.9 × 103	<0.001
AH5030	spoIIIGwtb	5.4 × 108	1.6 × 108	30.0
AH5031	spoIIIGE155K	9.0 × 108	2.9 × 108	32.0
AH5024	spoIIIJ spoIIIGwt	3.5 × 108	1.8 × 103	<0.001
AH5025	spoIIIJ spoIIIGE155K	3.4 × 108	2.9 × 103	<0.001
AH5011	spoIIIJ PspoIIIJ-spoIIIJ	5.5 × 108	2.2 × 108	40.0
AH5012	spoIIIJ PspoIIQ-spoIIIJ	4.3 × 108	2.6 × 108	61.0
AH5013	spoIIIJ PspoIID-spoIIIJ	1.2 × 108	5.9 × 106	5.0
AH2550	Wild type(pMK3)	7.9 × 108	4.5 × 108	57.0
AH5037	Wild type(pMS210)	1.5 × 108	6.0 × 106	4.0
AH5035	spoIIIJ(pMK3)	1.8 × 108	5.0 × 103	0.003
AH5036	spoIIIJ(pMS210)	1.5 × 108	5.8 × 106	3.9
AH62	spoIIIAΩHU24	2.3 × 107	9.0 × 102	<0.001
AH5020	PspoIIQ-spoIIIA	3.2 × 108	9.0 × 103	0.003
AH5029	PspoIID-spoIIIA	5.5 × 108	3.1 × 108	56.0

^aSporulation was measured 24 h after the onset of the process in liquid sporulation medium as described in Materials and Methods.

^bspoIIIGwt, wild-type spoIIIG.