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Upregulation of HIV-1 transcription by okadaic acid. 293 cells were grown to 50% confluency and transfected with a combination of HIV-1 LTR-LacZ and Tat expression vectors (panel A) or TAR deleted mutant of HIV-1 LTR-LacZ expression vector (panel B). Okadaic acid was added in increasing concentrations and the cells were assayed for β-galactosidase at 48 hours posttransfection. The results are expressed as a fold of transactivation.
