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. 2006 Oct 2;3:65. doi: 10.1186/1742-4690-3-65

Figure 3.

Figure 3

WD5 domain of LIS1 upregulates Tat mediated HIV-1 transcription. A. The WD5 domain and N-terminal domain of LIS1 were expressed in E. coli and extracted from the inclusion bodies as described in Methods. The dialyzed peptides were resolved on 12% SDS-PAGE gel and stained by Coumassie blue. B. 293 cells transfected with HIV-1 LTR-LacZ and Tat expression vectors and treated at 24 hrs posttransfection with WD5 domain or the N-terminal domain of LIS1. C, 293 cells transfected with TAR RNA-deleted HIV-1 LTR-LacZ vector and treated as in panel A. The results are presented as a fold of transactivation.