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. 2006 Oct 13;399(Pt 3):387–396. doi: 10.1042/BJ20060533

Figure 2. Competition of paionin-1 phage binding in ELISAs.

Figure 2

Binding of paionin-1 phage clone B to 10 nM PAI-1 from HT-1080 cells was detected in ELISA format 1 (see the Experimental section) in the presence of additions as indicated for each experiment. (A) Paionin-1: NH2-DVPCFGWCQDA-CO2H. Paionin-1, linear: NH2-DVPSFGWSQDA-CO2H. Bis-ANS and XR-5118: see [13]. (B) Effects from monoclonal anti-PAI-1 antibodies Mab-1, Mab-2 and Mab-6 and vitronectin on paionin-1 phage binding. Monoclonal anti-uPA Mab-2 was included as a control.