Table 1. Selected PAI-1-binding peptides.
Sequence alignment and EC50 for the PAI-1-binding peptides isolated by phage display. Consensus motifs are underlined. The frequency indicates the occurrence of each clone among the sequenced binders. The EC50 values were determined as the amount of PAI-1 required for half-maximal binding in ELISA format 1 (see the Experimental section).
| Clone | Sequence | Frequency | EC50 (nM) |
|---|---|---|---|
| A | CDPPCFGWCQDAC | 2 | 10 |
| B | CDVPCFGWCVAWC | 12 | 8.2 |
| C | CQGGCRVQCFGFC | 1 | 30 |
| F | CTGACPVVCFGFC | 1 | 17 |
| G | ILCFGMC | 1 | 58 |
| H | CEGVCRVSCFGWC | 3 | 6.0 |
| I | CDVPCFGWCVSWC | 1 | 13 |
| J | CVCFGYCRYEC | 1 | 9.9 |
| N | CHILCFGYCGDLC | 1 | 4.2 |
| O | CEGKCVVSCFGFC | 1 | 9.8 |