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(a) Effect of pH on the activity toward the synthetic substrate (●) and haemoglobin (○). (b) Effects of temperature on the activity (●) and stability (○). To measure the stability, the enzyme was pre-incubated for 1 h at different temperatures, then assayed at 37 °C. (c) Effect of incubation at pH 7.4 on the activity. (d) Effect of NaCl concentration on the activity.
