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. 2006 Oct 13;399(Pt 3):473–481. doi: 10.1042/BJ20060244

Figure 5. Effect of protein kinase and protein synthesis inhibitors on palmitoleate-induced glucose uptake in L6 myotubes.

Figure 5

L6 myotubes were pre-incubated in the absence or presence of (A) 100 nM wortmannin (Wort), 10 μM LY-294002 (LY), 100 nM rapamycin (Rap), 50 μM SB-415286 (SB4), 10 μM SB-203580 (SB2), 10 μM PD-98059 (PD), 5 μM cycloheximide (ChX) or 100 μM genistein (Gen), or (B) with either 0.1 or 1 μM Ro 31.8220, for 15 min prior to incubation with (+) or without (−) 0.75 mM palmitoleate (PO) for a further 4 h prior to assaying 2-deoxyglucose uptake. Values are means±S.E.M. of at least three separate experiments, each performed in triplicate. *Significant (P<0.05) increase compared with the appropriate untreated control; #significant inhibition (P<0.05) compared with palmitoleate treatment alone.