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. Author manuscript; available in PMC: 2007 Sep 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2006 Apr 17;215(2):158–167. doi: 10.1016/j.taap.2006.02.006

Figure 5.

Figure 5

Glutathione depletion in the presence/absence of parent drugs and their metabolites in NHDF cells: NHDF cells were seeded at a density of 5×105 cells/mL on a 6 well plate and incubated for 3 h with 1.5 mM of parent drug or metabolite. Media with NHDF cells alone were used to measure the basal GSH content, DMSO (0.25%) was used as the control, and DEM 0.4 mM was used as a positive control for GSH reduction. After 3 h incubation, the cells were washed with ice cold PBS and treated with 1 ml ice cold lysis buffer. Cells were lysed, extracted and analyzed for GSH content as described in Materials and Methods. GSH contents were determined using a standard curve generated from known concentrations and expressed as μmoles/g of protein. Data presented as mean (+SD) of four separate incubations for each condition. Results were analyzed using ANOVA with Holm-Sidak method for multiple pairwise comparisons. *p<0.05 compared to media, DMSO, DDS, SMX and SNOH; **p<0.05 compared to media, DMSO, DDS, and SMX; ***p<0.05 compared to media, DMSO, and SMX.