Figure 4. Tie-Tg mice exhibit enhanced B16-BL6 metastatic lung disease.
(A) Gross evaluation of lungs reveals that Tie-Tg mice develop substantially more and larger metastases 14 days after B16-BL6 melanoma injection (5 × 105 cells, i.v.). Images are representative of results obtained from WT and Tie-Tg (lines 2 and 4) mice. (B) H&E analysis with a ×10 objective of sections obtained from the lungs shown in A. Metastases are marked with arrows. (C) Metastasis was quantified by change in lung weight compared with a nonexposed control. Lung mass was determined in WT or Tg mice, either before (None) or after B16-BL6 tumorigenesis (B16; as in A). Total lung mass increased an average of 50 μg in WT and 250 μg in Tie-Tg lungs (P < 0.0001) compared with nonexposed controls. Data represent mice from both transgenic lines.