Figure 6. TLR4 deficiency prevents impaired insulin signaling in skeletal muscle caused by lipid infusion.

(A) Schematic diagram of lipid infusion and in vivo insulin signaling studies. (B) Mice preinfused with lipid for 8 hours were i.v. injected with 10 U/kg BW of human insulin (Ins) or saline. Muscle lysates were immunoprecipitated and then immunoblotted with antibodies as indicated. (C) Mice were infused with lipid for 8 hours. Muscle lysates were immunoblotted with antibodies as indicated. Ser, serine; Tyr, tyrosine.