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NPSN11 T-DNA insertion mutant. A, A T-DNA insertion in the NPSN11 (At2g35190) locus was identified from the sequence-tagged database created by the SALK Institute. The insertion was confirmed and characterized as described in “Materials and Methods.” B, Protein extracts from seedlings and flowers of representative siblings of known genotype were separated by SDS-PAGE and blotted with NPSN11 antiserum to show the absence of the NPSN11 protein in the homozygous npsn11 plants.
