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. 2002 Jun;129(2):530–539. doi: 10.1104/pp.003970

Figure 6.

Figure 6

NPSN11 interacts with the syntaxin KNOLLE. A, NPSN11 protein was immunoprecipitated from total membrane proteins prepared from 5-d-old Arabidopsis suspension-cultured cells. Total protein (1/300; T), protein eluate from preimmune column (1/10; Pre) or from NPSN11-immune column (1/10; IP) was separated by SDS-PAGE. NPSN11, SYP21, VTI12, and KNOLLE were detected by western blots. Note that the 41-kD band observed with the crude NPSN11 antiserum (indicated by an arrowhead) is not immunoprecipitated. The non-specific band recognized by anti-SYP21 is indicated by an asterisk. B, Immunoprecipitation of KNOLLE from 5-d-old Arabidopsis suspension cells using rabbit anti-KNOLLE cross-linked to protein A beads. Total membrane (1/300; T), flow-through (1/300; FT), and eluted protein (1/10; EL) were separated by SDS-PAGE and detected by western blot using antisera against KNOLLE or NPSN11.