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. 2002 Jun;129(2):625–637. doi: 10.1104/pp.001842

Figure 2.

Figure 2

Effect of light deprivation on MCCase expression. A, Changes in MCCase activity in 10-d-old Arabidopsis seedlings, which were initially grown under constant illumination and then transferred to darkness for the indicated time period. The data presented are means ± se from three replicates. B, Western-blot analysis of MCC-A and MCC-B accumulation in Arabidopsis, which were initially grown under constant illumination and then transferred to darkness for the indicated time period. Proteins were extracted from the samples and aliquots containing equal amounts of protein were fractionated by SDS-PAGE, followed by western-blot analysis with antiserum against MCC-A or MCC-B. C, Northern-blot analysis of MCC-A and MCC-B mRNA abundance in 10-d-old Arabidopsis seedlings, which were initially grown under constant illumination and then transferred to darkness for 4 d. Equal amounts of RNA (30 μg) isolated from each sample were fractionated by electrophoresis in a formaldehyde-containing agarose gel. The data presented in B and C were gathered from a single experiment; three replicates of this experiment gave similar results.