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. 2002 Jun;129(2):638–649. doi: 10.1104/pp.002675

Figure 1.

Figure 1

Expression of the Arabidopsis RHD3 gene. A through D, In situ RNA hybridization; E through X, RHD3 promoter::GUS reporter gene expression. A and B, Four-day-old wild-type root hybridized with RHD3 antisense RNA probe shows RHD3 transcript accumulation in the root elongation zone. Bar in A = 100 μm. Bar in B = 50 μm. C, Four-day-old rhd3-3 mutant root hybridized with RHD3 antisense RNA probe shows reduced RHD3 transcript accumulation. Bar = 100 μm. D, Four-day-old wild-type root incubated without RNA probe shows no hybridization signal. Bar = 100 μm. E and F, Four-day-old root from transgenic line harboring the X1 RHD3::GUS construct incubated for 30 min in X-Gluc substrate shows expression in the elongation zone. Bar in E = 100 μm. Bar in F = 50 μm. G and H, Four-day-old root apex (G) and mature root segment (H) from transgenic line harboring the X1 RHD3::GUS construct incubated for 4 h in X-Gluc substrate shows expression throughout the root. Bars = 100 μm. I and J, Four-day-old root elongation zone (I) and mature root segment (J) from transgenic line harboring the H1 RHD3::GUS construct incubated for 16 h in X-Gluc substrate shows preferential expression in the vascular tissue. Bar in I = 50 μm. Bar in J = 100 μm. K and L, Six-day-old seedling cotyledons from transgenic lines harboring the X1 (K) or H1 (L) RHD3::GUS constructs incubated for 16 h in X-Gluc substrate shows expression throughout the cotyledon (K) or in vascular tissue (L). Bars = 500 μm. M and N, Three-week-old rosette leaves from transgenic lines harboring the X1 (M) or H1 (N) RHD3::GUS constructs incubated for 16 h in X-Gluc substrate shows expression throughout the leaf (M) or in vascular tissue (N). Bars = 100 μm. O and P, Five-week-old stems from transgenic lines harboring the X1 (O) or H1 (P) RHD3::GUS constructs incubated for 16 h in X-Gluc substrate shows expression throughout the stem (O) or in vascular tissue (P). Bar in O = 200 μm. Bar in P = 100 μm. Q and R, Five-week-old pedicels from transgenic lines harboring the X1 (Q) or H1 (R) RHD3::GUS constructs incubated for 16 h in X-Gluc substrate shows expression throughout the pedicel (Q) or in particular cell files (R). Bar in Q = 200 μm. Bar in R = 100 μm. S through U, Five-week-old transgenic lines harboring the X1 RHD3::GUS constructs showing an immature flower (S), mature flower (T), or stigmatic tissue from mature flower (U) incubated for 16 h in X-Gluc substrate. Bars in S and T = 800 μm. Bar in U = 200 μm. V, Five-week-old sepal tissue from flowers of transgenic line harboring the H1 RHD3::GUS construct incubated for 16 h in X-Gluc substrate shows expression in vascular tissue. Bars = 100 μm. W and X, Embryos at curled cotyledon stage from transgenic lines harboring the X1 (W) or H1 (X) RHD3::GUS constructs incubated for 16 h in X-Gluc substrate shows expression throughout the embryo (W) or absent (X). Bars = 100 μm.