Figure 4.

Structure of RHD3 gene fusions containing or lacking the first intron. The H2-I construct contains the same upstream regulatory sequences as H2 except that the first intron is absent. For other constructs, a PCR fragment containing the first intron was added to the upstream or in the transcribed regions of various GUS reporter or RHD3 cDNA gene fusions. The orientation of the intron sequences is indicated by the arrows. The +1 symbol indicates the transcription initiation site. Details of the cloning procedures are described in “Materials and Methods.”