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Cytosolic AAT2 activity in leaves and siliques. Cytosolic AAT2 activity occurs in both leaves and siliques in situ. Levels of Asp and Asp-related amino acids in siliques represent those made by AAT2 in siliques and possibly transported amino acids from leaves. Cytosolic AAT2, in the forward direction, utilizes Glu and oxaloacetate (OAA), leading to the synthesis of Asp, the precursor for Asn synthesis by AS, and alpha-ketoglutarate (α-KG).
