Figure 3.

RSY3 genomic DNA sequence and its predicted protein. A, Portion of the genomic DNA sequence of the RSY3 gene. Introns are in lowercase letters, and the exons and the untranslated sequences are in uppercase letters. Nucleotides highlighted in blue are missing or are different from the reported sequence based on a Columbia background (see sequence of predicted gene MOB24.12 in accession AB020746). The predicted amino acids are given above the coding sequence. Amino acids highlighted in red are those that differ from the predicted amino acid sequence in the RSY3 locus of the Columbia ecotype. B, Alignment of the RSY3 protein to other proteins with similar domains. The amino acid sequences derived from the predicted coding sequences were aligned using the AlignX program of Vector NTI software. The highlighted domains (I, II, and III) were subsequently found using the AlignX Block program of the same software. Domain I, highlighted in gray, is the putative ATP-binding domain. Consensus core regions (as cited in the text) within the three domains are highlighted with a bold line above the sequences. The proteins included in the above alignment have the following accession numbers: Porphyra purpurea (AAC08269), Synechocystis sp (BAA10210), Escherichia coli (BAA77863), Heliobacillus mobilis (AAC84036), Mycobacterium tuberculosis (AAK48088), Rickettsia prowazekii (CAA14513), Bacillus subtilis (BAA05302), Xylella fastidiosia (AAF83469), Mesorhizobium loti (BAB50680), Bacillus halodurans (BAB03802), Bradyrhizobium japonicum (BAB50680), Saccharomyces pombe (CAA94698), E. coli GuaA (AAG57618), B. halodurans GuaA (Q9KF78), B. subtilis GuaA (P29727), Arabidopsis RSY3-like (AAC16077), and fruit fly (Drosophila melanogaster) predicted protein (AAF58991). The accession number for the RSY3 genomic sequence is AY077630.