Figure 1.

Synthesis of [³²P]MRS2500. MRS2608 (5 μl of a 10 mM solution) was combined with 1.5 μl of 10 × reaction buffer, 1 mCi of [γ³²P]ATP (7 μl, 0.16 nmol, 150 mCi ml⁻¹), and 2 μl (20 U) of 3′-phosphatase-free polynucleotide kinase. The sample was mixed by pipetting and incubated at 37°C for 1 h. The entire reaction volume was then injected onto a Luna 5μ C18(2) column for purification under mobile phase conditions as described in Methods.