Figure 1.

Phosphorylation of purified EGFR in the presence or absence of peptides. (a) Peptides including Y1148 (pY, phosphorylated tyrosine), (b) peptides including Y1173 and the control peptide, Ac-LPFFD-NH₂, (c) a peptide including Y1068, and (d) peptides including Y992. EGFR was incubated with or without peptides for 5 min at 37°C in the buffer containing 0.2 mM of ATP, except open bars shown in (c), where 0.02 mM of ATP was used. Results displayed on the top panels represent typical immunoblots (IB). ^*P<0.05, ^**P<0.01 versus EGF-stimulated tyrosine phosphorylation at 5-min incubation without peptides; n=4 for each lane.