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Phosphorylation of purified EGFR in the presence or absence of AG1478. EGFR was incubated in the buffer with or without AG1478 for 5 min at 37°C with 0.2 mM of ATP (closed bars) or with 0.02 mM of ATP (open bars). Results displayed on the top panels represent typical immunoblots (IB). **P<0.01 versus EGF-stimulated Tyr phosphorylation at 5-min incubation without AG1478; n=4 for each lane.
