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Effects of tyrosine to alanine mutation in DYQQD, ENAEYLR, and VPEYINQ on the inhibitory potencies for autophosphorylation of EGFR. EGFR was incubated in the buffer with or without peptides for 5 min at 37°C. Results displayed on the top panels represent typical immunoblots (IB). **P<0.01 versus EGF-stimulated tyrosine phosphorylation at 5-min incubation without peptides; n=4 for each lane.
