Table I.
Spectral properties of the various LOV-domain fusion proteins
Construct | Wavelength of Peak | Extinction at Peak Wavelength | Kppr (× 102) | Photoproduct at Photoequilibrium | Relative Quantum Efficiencya |
---|---|---|---|---|---|
nm | 1 mol−1 cm−1 | s−1 | % | ||
Atb phot1 LOV1 | 448.5 | 13,900 | 0.38 | 37 | 0.035 |
At phot1 LOV2 | 446.5 | 14,300 | 3.9 | 91 | 0.34 |
At phot1 LOV1+2 | 447.5 | 13,700 | 1.9 | 55 | |
At phot2 LOV1 | 447.5 | 14,700 | 1.5 | 62 | 0.13 |
At phot2 LOV2 | 445.5 | 13,500 | 2.9 | 52 | 0.27 |
At phot2 LOV1+2 | 446.5 | 13,400 | 2.4 | 55 | |
Os phot1 LOV1 | 449.5 | 11,200 | 0.23 | 33 | 0.026 |
Os phot1 LOV2 | 446.5 | 15,200 | 3.7 | 87 | 0.30 |
Os phot1 LOV1+2 | 448.0 | 11,800 | 1.6 | 41 | |
Os phot2 LOV1 | 448.0 | 11,100 | 2.9 | 83 | 0.33 |
Os phot2 LOV2 | 446.5 | 13,300 | 3.1 | 81 | 0.29 |
Os phot2 LOV1+2 | 447.0 | 13,300 | 2.9 | 77 | |
Cr LOV1 | 447.0 | 13,800 | 3.3 | 73 | 0.30 |
Cr LOV2 | 445.5 | 13,300 | 3.7 | 69 | 0.35 |
Cr LOV1+2 | 446.5 | 13,200 | 3.6 | 75 |
Approximate efficiencies (Q) were calculated from the equation Q = Kppf ε−1 I−1, where Kppf is the initial rate of photoproduct formation in s−1, ε is the molar extinction coefficient in 1 mol−1 cm−1, and I is the fluence rate of the actinic light in μmol m−2 s−1.
At, Arabidopsis; Os, rice; Cr, C. reinhardtii.