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. 2006 Jan 9;147(4):422–429. doi: 10.1038/sj.bjp.0706624

Figure 1.

Figure 1

Under the routine conditions of [35S]GTPγS autoradiography, SCH-202676 has no effect on receptor-dependent G protein activity in rat brain cryostat sections. [35S]GTPγS autoradiography of coronal brain sections was conducted using a three-step protocol with 10−6 M DPCPX present throughout steps 2 and 3, as detailed in the Methods section. SCH-202676 was present at the indicated concentrations together with 1 mM DTT during the [35S]GTPγS labeling (step 3). The GPCR agonists 2-methylthio ADP (2MeSADP), lysophosphatidic acid (LPA), CP-55,940 and sphingosine 1-phosphate (S1P) were present during step 3 to stimulate purinergic P2Y12, lysophosphatidic acid LPA1, cannabinoid CB1 and sphingosine 1-phosphate S1P1 receptors, respectively. In the control panel (left), the anatomical loci where the receptor agonists typically activate G proteins at this coronal plane are indicated. Note the apparent lack of effect of SCH-202676 on nonspecific, basal or agonist-stimulated responses. Abbreviations: cc, corpus callosum; Cx, cerebral cortex; EP, entopeduncular nucleus fi; fimbria of the hippocampus; Hip, hippocampus, Hy, hypothalamus; opt, optic tract. Scale bar=2 mm.