Table II.
Arabidopsis polypeptides whose abundance was significantly higher in dry mature ga1 seeds than in dry mature WT seeds
| No. | Experimental Molecular Mass | Experimental pI | Arabidopsis Protein Name | Coverage | Theoretical Molecular Mass | Theoretical pI | Accession No. | Relative Abundancea |
|---|---|---|---|---|---|---|---|---|
| kD | % | kD | ||||||
| 169 | 47.20 | 6.7 | 12S-1 Seed storage protein precursor | 15 | 49.99 | 7.26 | P15455 | >200 |
| 151 | 45.59 | 5.39 | Similar to 12S seed storage precursor | 23 | 49.67 | 5.47 | 4204298 | 3.5 ± 0.1 |
| 177 | 42.94 | 6.12 | 12S-2 Seed storage protein precursor | 14 | 50.64 | 6.77 | 112682 | 2.4 ± 0.1 |
| 71 | 50.44 | 7.67 | 12S-1 Seed storage protein precursor | 22 | 49.99 | 7.26 | P15455 | 2.4 ± 0.1 |
| 70 | 49.43 | 7.19 | 12S-1 Seed storage protein precursor | 61 | 49.99 | 7.26 | P15455 | 2.0 ± 0.1 |
| 69 | 43.67 | 6.29 | 12S-2 Seed storage protein precursor | 18 | 48.03 | 6.56 | P15456 | 1.8 ± 0.1 |
a
Data obtained from densitometric analysis of individual spots (see Fig. 1): normalized spot volume in the mature dry ga1 seeds divided by the normalized spot volume in the dry mature WT seeds ± sd (n = 3); >200 means that the accumulation level of the corresponding protein in the dry mature WT seeds was close to background.