Table IV.
Arabidopsis proteins whose level increased during radicle protrusion (type-3 proteins) with the WT seeds incubated for 2 d in water
| Protein Type | No. | Experimental Molecular Mass | Experimental pI | Arabidopsis Protein Name | Coverage | Theoretical Molecular Mass | Theoretical pI | Accession No. | Relative Abundancea |
|---|---|---|---|---|---|---|---|---|---|
| kD | % | kD | |||||||
| 3b | 63 | 57.09 | 6.11 | Isocitrate lyase | 30 | 50.42 | 6.29 | 113024 | >200 |
| 3b | 58 | 36.28 | 5.49 | Jasmonate-inducible MBP | 41 | 32.16 | 5.46 | 9279641 | >200 |
| 3c | 62 | 43.25 | 5.67 | Ado-Met synthetase 2 | 30 | 43.25 | 5.67 | 127045 | >200 |
| 3b | 67 | 63.25 | 6.47 | Thioglucosidase (myrosinase) | PSD | 60.26 | 6.91 | S57621 | >200 |
| 3c | 47 | 66.57 | 5.33 | Cytosolic phosphoglyceromutase | 17 | 60.67 | 5.32 | 11133828 | >200 |
| 3b | 49 | 42.67 | 5.51 | Ado-Met synthetase | 19 | 42.79 | 5.51 | 9229983 | 18 ± 1 |
| 3d | 65 | 29.65 | 5.82 | α-Subunit of CRA1 cruciferin | 24 | 29.23 | 6.49 | 81604 | 8.5 ± 0.1 |
| 3b | 50 | 42.94 | 5.43 | Chloroplastic translation elongation factor EF-Tu (mature form) | 23 | 44.72 | 5.32 | S09152 | 5 ± 1 |
| 3c | 179 | 37.67 | 5.88 | Mitochondrial malate dehydrogenase | PSD | 33.9 | 6.00 | 11133715 | 4.0 ± 0.3 |
| 3c | 73 | 42.28 | 6.47 | β-Glucosidase | 18 | 60.01 | 6.20 | 9294684 | 4.0 ± 0.4 |
| 3b | 66 | 62.82 | 6.33 | Jasmonate-inducible protein | 16 | 62.00 | 6.00 | 8777418 | 3.1 ± 0.2 |
PSD, Protein identified by MALDI-TOF and PSD analyses.
a
Data obtained from densitometric analysis of individual spots (see Figs. 2C and 6): normalized spot volume in the 2-d imbibed seeds divided by the normalized spot volume in the 1-d imbibed seeds ± sd (n = 3); >200 means that the accumulation level of the corresponding protein in the 1-d imbibed seeds was close to background.
b
Polypeptides identified by Gallardo et al. (2001).
c
Polypeptides identified by MALDI-TOF and/or PSD analyses in the present study.