Table 1.
Effect of YM-254890 on platelet aggregation induced by various agonists in human PRP and washed human platelets
| Agonist | IC50 (μM) |
|---|---|
|
PRP | |
| ADP (5 μM) |
0.39±0.06 |
| Collagen (0.25–0.5 μg ml−1) |
0.15±0.05 |
| TRAP (1–5 μM) |
0.71±0.23 |
| Arachidonic acid (0.75 mM) |
0.25±0.04 |
| U46619 (1–3 μM) |
0.34±0.11 |
| PMA (1–5 μM) |
>100 |
| Ristocetin (1.5 mg ml−1) |
>100 |
| SIPA |
0.57±0.07 |
| | |
|
Washed platelets | |
| Thapsigargin (2.5–5 μM) |
>100 |
| A23187 (2.5–10 μM) | >100 |
The IC50 values were calculated from the concentration–inhibition curves for each experiment. Data represent the mean±s.e.m. of three or four separate experiments.