Table II.
Real-time PCR primers used for evaluation of steady-state mRNA levels of APX and actin 2 (ACT2)
Primer
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Protein Name and Intracellular Localization | mRNA GenBank Accession No. | Name | Sequence | Position to Stop Codon |
APX1, cytosolic | X59600 | Apx1A | 5′-GTCCATTCGGAACAATGAGGTTTGAC-3′ | −587 bp |
Apx1B | 5′-GTGGGCACCAGATAAAGCGACAAT-3′ | −262 bp | ||
APX2, cytosolic | X98275 | Apx2A | 5′-TGATGTGAAGACGAAGACAGGAGGAC-3′ | −610 bp |
Apx2B | 5′-CCCATCCGACCAAACACATCTCTTA-3′ | −305 bp | ||
APX3, microsomal | X98003 | Apx3A | 5′-CCCAAAATCACATACGCAGACCTGTA-3′ | −603 bp |
X98276 | Apx3B | 5′-AGTTGTCAAACTTCAGCGGCTCTTG-3′ | −306 bp | |
APX4, microsomal | AF441713 | Apx4A | 5′-CTACTAAATCCGGGGGAGCCAATG-3′ | −659 bp |
Apx4B | 5′-CTCTGTTGCATCACTCCTTCCAAAAT-3′ | −337 bp | ||
APX5, microsomal | AF441714 | Apx5A | 5′-AGCTAAACCGTCCACACAACAAAGGT-3′ | −653 bp |
Apx5B | 5′-GTCCCAAAGTGTGACCTCCAGAGAGA-3′ | −354 bp | ||
APX6, cytosolic | AV555486 | Apx6A | 5′-TGCAAAACGAAATAAGGAAAGTGGTG-3′ | −497 bp |
AI995973 | Apx6B | 5′-CACTCAGGGTTTCTGGAGGTAGCTTG-3′ | −255 bp | |
sAPX, chloroplastic stromal | X98925 | ApxSA | 5′-TGCTAATGCTGGTCTTGTGAATGCTT-3′ | −625 bp |
ApxSB | 5′-CCACTACGTTCTGGCCTAGATCTTCC-3′ | −209 bp | ||
tAPX, chloroplastic thylakoidal | X98926 | ApxTA | 5′-CAGAATGGGACTTGATGACAAGGAAA-3′ | −604 bp |
ApxTB | 5′-ATGCAGCCACATCTTCAGCATACTTC-3′ | −306 bp | ||
ACT2 | U41998 | Act2A | 5′-ACCTTGCTGGACGTGACCTTACTGAT-3′ | −592 bp |
Act2B | 5′-GTTGTCTCGTGGATTCCAGCAGCTT-3′ | −295 bp | ||
Act2C | 5′-ATTCAGATGCCCAGAAGTCTTGTTCC-3′ | −366 bp | ||
Act2D | 5′-ACCACCGATCCAGACACTGTACTTCC-3′ | −99 bp |