Figure 6.

Magnolol treatment suppresses IL-6-induced cyclin D1 and MCP-1 promoter activities. Promoter construct of cyclin D1 (1745 bps) or MCP-1 (540 bps) containing luciferase as a reporter gene was cotransfected with pSV-β-galactosidase plasmid into ECs. ECs were pretreated with Mag at indicated concentration for 1 h followed with the stimulation of IL-6 (10 ng ml⁻¹) for 16 h in the presence of Mag. ECs treated with VEGF (10 ng ml⁻¹) or PMA (100 ng ml⁻¹) were used as positive controls for cyclin D1 and MCP-1, respectively. Luciferase activities were normalized with β-galactosidase and results were expressed as mean±s.e. from three independent experiments. ^*P<0.05 vs IL-6-treated ECs without Mag.