Developmental analysis of MGU assembly and positioning in wild-type pollen. a, Polarized microspore; b, early bicellular pollen; c, late bicellular stage; d and e, early tricellular pollen; f, mature pollen. g, Graph showing the frequency of typical wild-type phenotypes (illustrated) at the early tricellular (−2), late tricellular (−1), and mature pollen (+1) stages. More than 400 spores were counted at each stage.