Table 1.
Effect of B. Jararaca venom and GL and/or antibothropic serum treatments on ex vivo clotting tests
|
Animal group |
Clotting times |
|||||
|---|---|---|---|---|---|---|
| aPTT (s) | PT (s) | |||||
| Control |
23.5±1.8 |
25.7±2.8 |
||||
| |
|
|
|
|
|
|
| |
Time after venom administration (min) |
|||||
| Treated with |
10 |
30 |
120 |
10 |
30 |
120 |
| Venom1 |
C |
NC |
NC |
C |
NC |
NC |
| Venom1+GL2 |
25.4±0.4 |
28.2±1.5 |
19.1±2.6 |
33.8±9.3 |
32.3±0.8 |
42.6±8.0 |
| Venom1+As3 |
31.9±5.4 |
29.2±3.6 |
36.7±12.6 |
35.5±3.7 |
37.1±6.2 |
50.8±2.2 |
| Venom1+GL2+As4 | C | 22.4±1.3 | 38.5±4.3 | C | 26.6±2.6 | 52.2±2.5 |
PT and APTT clotting times were measured as described in Methods. Citrated plasma samples were taken at the indicated times from animals treated as described: 1B. jararaca venom, 100 μg kg−1; 2GL, 40 mg kg−1, antibothropic serum, 3As, 27 μl kg−1 or 4As, 3 μl kg−1. Data show mean±s.d. of at least five animals.
C means that blood clotted before tests could be performed.
NC means that plasma did not clot until 150 s.