Figure 3.

GLP disrupted tube formation by HMEC-1 cells in Matrigel. HMEC-1 was seeded in Matrigel-coated 96-well plates. (a) HMEC-1 cells formed a complete mesh of tubes within 8 h on Matrigel. After treatment with GLP, cells stuck out short protrusions and aggregated into small masses (b, 0.313 mg ml⁻¹ GLP) or showed no differentiation morphology (c, 1.25 mg ml⁻¹ GLP). (d) In five random fields, tubes in enclosed networks were counted. The mean of three independent experiments are presented, error bars indicate standard deviation. Magnification: 40-fold. ^**P<0.01, GLP group vs control group.