Figure 2.
Unrooted, bootstrapped tree of NRT2 proteins. ClustalW was used to create an alignment of the entire sequence of amino acids that was then bootstrapped (n = 1,000) to create the final tree using the Philip package. Values indicate the number of times (in percent) that each branch topology was found during bootstrap analysis. D, Dicotyledons; M, monocotyledons. GenBank protein ID numbers are: AtNRT2.1, AAC64170; AtNRT2.2, AAC35884; AtNRT2.3, BAB10099; AtNRT2.4, BAB10098; AtNRT2.5, AAF78499; AtNRT2.6, CAB89321; AtNRT2.7, CAB87624; Nicotiana plumbaginifolia NpNRT2.1, CAA69387; Lotus japonicus LjNRT2, CAC35729; soybean (Glycine max) GmNRT2, AAC09320; rice (Oryza sativa) OsNRT2, BAA33382; barley (Hordeum vulgare) HvNRT2.1, AAC49531; wheat (Triticum aestivum) TaNRT2.1, AAK19519; Chlamydomonas reinhardtii CrNRT2.1, CAA80925; Aspergillus nidulans CRNA, AAA62125; Hansenula polymorpha YNT1, CAA93631; and E. coli NARK, CAA34126.