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The sup45-sl23ts mutation is lethal in the strain 33G-D373-rSL23-ΔS35, carrying the SUP35-C or SUP35-MC alleles. The LEU2 plasmids with SUP35-C or SUP35-MC can not replace the resident URA3 plasmid pRS316-SUP35 carrying wild type SUP35 in the strain 33G-D373-rSL23-ΔS35. Transformants were incubated on 5FOA-containing medium for 3 days. Plasmids: SUP35-C, pRS315-SUP35C; SUP35-MC, pRS315-SUP35MC; SUP35, pRS315-SUP35; empty vector, pRS315.
