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. 2006 Oct 11;7:34. doi: 10.1186/1471-2199-7-34

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Copyright © 2006 Urakov et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Compensatory mutations in plasmid SUP35-C restore viability of the strain carrying the sup45-36^tsand SUP35-C alleles. The sup45-36^tsmutant 33G-D373-r36-ΔS35 disrupted for SUP35 and carrying the SUP35 URA3 pRS316-SUP35 plasmid was transformed with either one of the LEU2 plasmids pRS315-SUP35 (SUP35), pRS315-SUP35C (SUP35-C), pRS315-SUP35-C11 (SUP35-C11), pRS315-SUP35-C14 (SUP35-C14), pRS315-SUP35-C42 (SUP35-C42) or pRS315 (empty vector). Transformants were incubated on 5FOA-containing medium for 3 days.