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Kinetics of S segment RNA synthesis during hantavirus infection. HUVECs were mock infected or infected with NY-1V, HTNV, or PHV (MOI of 1). S segment RNA levels were determined by quantitative real-time PCR using hantavirus S segment-specific primers 1 to 4 days postinfection, and responses from duplicates were normalized to GAPDH mRNA levels. Experiments were performed twice with similar results, and mRNA levels are expressed as the change (n-fold) from 1-day levels.
