FIG. 3.

Western blot analysis of N protein expression. HUVECs were infected with NY-1V, HTNV, or PHV (MOI of 1) or mock infected (C). Cells were lysed at 1 hour or 1 to 5 days postinfection as indicated. Total protein levels were determined, and an equivalent amount of whole-cell lysate was separated by 10% SDS-polyacrylamide gel electrophoresis. Proteins were detected by Western blotting using anti-nucleocapsid polyclonal rabbit antibody or anti-tubulin monoclonal antibody (Sigma), species-specific secondary antibodies (HRP conjugated), and enhanced chemiluminescence (Amersham).