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Low-pH-triggered cell-cell fusion assays. BS-C-1 cells were infected with vF9Li in the presence or absence of IPTG. At 18 h after infection, the cells were treated for 5 min with pH 7.4 or pH 5.3 buffer at 37°C and then returned to normal medium. After 18 h, the cells were fixed, permeabilized, and stained with DAPI. Cells were visualized by phase-contrast microscopy, while DNA (blue) and GFP (green) staining were visualized by fluorescence microscopy.
