FIG. 7.
Polypeptide composition of purified F9+ and F9− virions. Purified MVs were obtained by sedimentation through two successive 36% sucrose cushions and a 25 to 40% sucrose gradient. Equal numbers of VACV WR, F9+, and F9− virions were solubilized with SDS and separated by SDS-PAGE in 4 to 12% polyacrylamide Bis-Tris gels. (A) Silver stain. The masses in kilodaltons are indicated on the left. (B) Western blot probed with anti-F9, anti-L1, anti-A21, and anti-A28 antibodies as indicated.