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. 2006 Oct;80(19):9511–9518. doi: 10.1128/JVI.01035-06

FIG. 2.

FIG. 2.

Homotypic and heterotypic interactions of VSV P protein in the yeast two-hybrid assay. (A) The yeast AH109 strains (type a) expressing BD-baits were mated with the yeast Y187 strains (type α) expressing AD-preys in the indicated combinations. The yeast diploid cells (AH109/Y187) were plated onto the selective plates with SD/−Trp/−Leu, to confirm the presence of plasmids for BD-baits and AD-preys (left panel), and SD/−Trp/−Leu/−His/−Ade/+X-α-Gal (X-a-Gal), to detect interactions between BD-baits and AD-preys (right panel). Yeast coexpressing interacting proteins which activate the expression of reporter genes (HIS, ADE2, and MEL1) grows as blue colonies on the SD/−Trp/−Leu/−His/−Ade/+X-α-Gal plates. The diploid cells coexpressing AD-T and BD-p53 were used as positive controls. Experiments were repeated at least three times for consistency. (B) Plasmids encoding Pind and mutants were fused to AD (AD-preys). Plasmids encoding Pind and Pnj were fused to BD (BD-baits). The yeast two-hybrid assay was performed as described for panel A.