FIG. 3.

Mapping of the self-association domain of Pind protein. Myc-Pind and Pind or its mutants tagged with HA were coexpressed in HeLa cells as indicated. Cell lysates were assayed by Western blotting (WB) using anti-Myc and anti-HA monoclonal antibodies to confirm protein expression (A and B, left panel). Immunoprecipitation (IP) was performed using anti-Myc polyclonal antibodies, and the immune complexes were detected by anti-Myc and anti-HA monoclonal antibodies, respectively (right panels of panels A and B).